2-CHLOROETHANOL AND PROTEIN SHELL OF BACTERIOPHAGE fr
نویسندگان
چکیده
In mixtures of 1 volume of buffer and 2 volumes of 2-chloroethanol, the icosahedral bacterio phage fr is split into RNA and monomeric protein subunits. After removal of the RNA and after replacement of the organic solvent by water, viruslike particles can be obtained by dialysis of the protein against neutral buffers of high ionic strength, whereas multishell particles are formed in buffers of low ionic strength. All results achieved by the use of 2-chloroethanol are very similar to those obtained using acetic acid.
منابع مشابه
Identification of chloroacetaldehyde dehydrogenase involved in 1,2-dichloroethane degradation.
The degradation of 1,2-dichloroethane and 2-chloroethanol by Xanthobacter autotrophicus GJ10 proceeds via chloroacetaldehyde, a reactive and potentially toxic intermediate. The organism produced at least three different aldehyde dehydrogenases, of which one is plasmid encoded. Two mutants of strain GJ10, designated GJ10M30 and GJ10M41, could no longer grow on 2-chloroethanol and were found to l...
متن کاملProtein conformation in cell membrane preparations as studied by optical rotatory dispersion and circular dichroism.
To elucidate the basic structure of biological membranes, experimental evidence concerning the detailed molecular organization of the proteins of membranes is required. This evidence has been almost totally lacking or ambiguous up to the present time. The Cotton effects due to the peptide bond ultraviolet absorption bands, as studied by ORD and CD, have recently proved extremely valuable in cha...
متن کاملCell-specific targeting by engineered M13 bacteriophage expressing VEGFR2 nanobody
Objective(s): Filamentous bacteriophage M13 was genetically engineered to specifically target mammalian cells for gene delivery purpose. Materials and Methods: A vascular endothelial growth factor receptor 2 (VEGFR2)-specific nanobody was genetically fused to the capsid gene III of M13 bacteriophage (pHEN4/3VGR19). A mammalian expression construct containing Cop-green fluorescent protein (Cop-G...
متن کاملFouling mechanisms during protein microfiltration: The effects of protein structure and filtration pressure on polypropylene microporous membrane performance
A polypropylene microporous membrane (PPMM) was fabricated by thermally induced phase separation (TIPS) method. The effects of protein size and structure as well as filtration pressure on the membrane performance and fouling mechanisms were investigated using two different proteins, bovine serum albumin (BSA) and collagen, in dead-end filtration setup. Obtained results showed that, for each pro...
متن کامل2-Chloroethanol Induced Upregulation of Matrix Metalloproteinase-2 in Primary Cultured Rat Astrocytes Via MAPK Signal Pathways
This study was to explore the mechanisms underlying 1,2-dichloroethane (1,2-DCE) induced brain edema by focusing on alteration of matrix metalloproteinase-2 (MMP-2) in rat astrocytes induced by 2-chloroethanol (2-CE), an intermediate metabolite of 1,2-DCE in vivo. Protein and mRNA levels of MMP-2, and the phosphorylated protein levels of p38 MAPK (p-p38), extracellular signal regulated protein ...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
دوره شماره
صفحات -
تاریخ انتشار 2013